Does bone marrow-derived mesenchymal stem cell transfusion prevent antisperm antibody production after traumatic testis rupture?
Urology
; 84(1): 82-6, 2014 Jul.
Article
in En
| MEDLINE
| ID: mdl-24797037
ABSTRACT
OBJECTIVE:
To determine whether transfusion of mesenchymal stem cells (MSCs) could prevent humoral immune response and autoimmunization against sperms after traumatic testis rupture.METHODS:
Immunomodulatory properties of MSCs have been evaluated by a prospective cohort on 50 adult BALB/c mice. In each interventional arms of study, controlled testis rupture and surgical repair were exerted. In addition to tissue repair, single dose of 5×10(5) MSCs labeled by green fluorescent protein was delivered intravenously to 20 cases (cell therapy group). After euthanizing, seroconversion of antisperm antibody (ASA) was compared between 2 interventional groups as response of humoral immune system. Lung and testis tissues were examined for green fluorescent protein-positive cells to assess whether presence of stem cells is correlated with seroconversion rates.RESULTS:
Six cases had been lost during the study. Fourteen of 16 mice in cell therapy control group formed ASA (87.5%) but 6 of 18 mice (33.3%) in cell therapy group were immunized and formed ASA (P=.002). Transplanted cells were traced in lungs of 55% (n=10) of cell therapy group and none were found in trauma site. Small volume of mice blood was our main limitation to trace seroconversion or quantitative measurement of ASA in each case.CONCLUSION:
In this in vivo model of autoimmune infertility, bone marrow-derived MSC transfusion showed immunosuppressive effects on antibody production. Considering immunomodulatory properties of MSCs even in allogeneic settings, novel clinical application should be investigated further.
Full text:
1
Database:
MEDLINE
Main subject:
Autoantibodies
/
Autoimmune Diseases
/
Spermatozoa
/
Testis
/
Mesenchymal Stem Cell Transplantation
/
Antibody Formation
Type of study:
Observational_studies
Limits:
Animals
Language:
En
Journal:
Urology
Year:
2014
Type:
Article
Affiliation country:
Iran