Secretion of cholesteryl ester transfer protein-lipoprotein complexes by human HepG2 hepatocytes.
Atherosclerosis
; 77(1): 77-82, 1989 May.
Article
in En
| MEDLINE
| ID: mdl-2497752
ABSTRACT
We have employed immunoaffinity chromatography to characterize the distribution of cholesteryl ester transfer activity in particles secreted by HepG2 hepatocytes. HepG2-secreted cholesteryl ester transfer activity is associated with apoprotein (apo) A-I (58%) as well as apo A-II (55%), and is not associated with apo B or E. In contrast, our previous studies have shown that most (88%) cholesteryl ester transfer activity in human plasma is associated with apo A-I whereas very little (7%) is associated with apo A-II. Thus, the distribution of cholesteryl ester transfer activity in plasma particles likely reflects active remodeling of nascent particles in the plasma compartment. Further data suggested that HepG2 cells secrete a lipid transfer inhibitor activity which is associated with apo E-containing lipoprotein particles. This inhibitory activity is heat labile.
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Database:
MEDLINE
Main subject:
Apolipoproteins
/
Glycoproteins
/
Carrier Proteins
Limits:
Humans
Language:
En
Journal:
Atherosclerosis
Year:
1989
Type:
Article