Expression of recombinant alpha and beta tubulins from the yew Taxus cuspidata and analysis of the microtubule assembly in the presence of taxol.

Kudo, Yuma; Abe, Akihiro; Ito, Kumiko; Cho, Yuko; Yotsu-Yamashita, Mari; Konoki, Keiichi

Kudo, Yuma; Abe, Akihiro; Ito, Kumiko; Cho, Yuko; Yotsu-Yamashita, Mari; Konoki, Keiichi.

Affiliation

Kudo Y; a Graduate School of Agricultural Science , Tohoku University , Sendai , Japan.

Biosci Biotechnol Biochem ; 78(11): 1887-94, 2014.

Article in En | MEDLINE | ID: mdl-25070196

Subject(s)

Gene Expression Regulation, Plant; Microtubules/drug effects; Microtubules/metabolism; Paclitaxel/pharmacology; Taxus/chemistry; Taxus/genetics; Tubulin/genetics; Amino Acid Substitution; Cloning, Molecular; Consensus Sequence; HEK293 Cells; Humans; Molecular Structure; Protein Binding/drug effects; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment; Taxus/drug effects; Taxus/metabolism; Tubulin/chemistry; Tubulin/metabolism; Tubulin Modulators/pharmacology

Key words

microtubule; self-resistance; taxol; tubulin

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Full text: 1 Database: MEDLINE Main subject: Tubulin / Paclitaxel / Gene Expression Regulation, Plant / Taxus / Microtubules Limits: Humans Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2014 Type: Article Affiliation country: Japan

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