[Induction on callus culture and regeneration of Orostachyis fimbriatae].
Zhong Yao Cai
; 37(1): 1-4, 2014 Jan.
Article
in Zh
| MEDLINE
| ID: mdl-25090694
ABSTRACT
OBJECTIVE:
To explore the effects of different hormonal combinations on induction, proliferation and differentiation of Orostachyis fimbriatae callus culture.METHODS:
Aseptic seedling leaves were used as explants,the different concentrations of IAA,NAA, 6-BA and KT on induction proliferation of callus were optimized by orthogonal test to explore the optimum medium for differentiation of callus by tissue culture techniques.RESULTS:
The best medium for induction was MS + IAA 1.0 mg/L + NAA 0.5 mg/L + KT 1.0 mg/L, and the best hormonal combination for proliferation was MS + IAA 0.5 mg/L + 6-BA 0.5 mg/I. + KT 1.0 mg/L. The best medium for differentiation was MS + IAA 0.1 mg/L + KT 2.0 mg/L, and 1/2MS + IAA 0.2 mg/L was the optimum medium for rooting culture.CONCLUSION:
The system of regeneration of Orostachyis fimbriatae is establishd by tissue culture techniques in this study.
Search on Google
Database:
MEDLINE
Main subject:
Plant Growth Regulators
/
Regeneration
/
Crassulaceae
/
Tissue Culture Techniques
Language:
Zh
Journal:
Zhong Yao Cai
Journal subject:
TERAPIAS COMPLEMENTARES
Year:
2014
Type:
Article