Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays.

Zhou, Ying; Asahara, Haruichi; Schneider, Nils; Dranchak, Patricia; Inglese, James; Chong, Shaorong

Zhou, Ying; Asahara, Haruichi; Schneider, Nils; Dranchak, Patricia; Inglese, James; Chong, Shaorong.

Affiliation

Zhou Y; New England Biolabs, Inc. 240 County Road, Ipswich, Massachusetts 01938, United States.

J Am Chem Soc ; 136(40): 14031-8, 2014 Oct 08.

Article in En | MEDLINE | ID: mdl-25188838

Subject(s)

Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genetic Engineering/methods; Transcription, Genetic; Two-Hybrid System Techniques; Cell-Free System; DNA/metabolism; DNA-Directed RNA Polymerases/metabolism; Drug Evaluation, Preclinical; Early Growth Response Protein 1/metabolism; Genes, Reporter/genetics; High-Throughput Screening Assays; RNA/metabolism; Small Molecule Libraries/pharmacology; Viral Proteins/metabolism

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Full text: 1 Database: MEDLINE Main subject: Transcription, Genetic / Genetic Engineering / Gene Expression Regulation, Bacterial / Two-Hybrid System Techniques / Escherichia coli Language: En Journal: J Am Chem Soc Year: 2014 Type: Article Affiliation country: United States

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