IL-27 is required for shaping the magnitude, affinity distribution, and memory of T cells responding to subunit immunization.
Proc Natl Acad Sci U S A
; 111(46): 16472-7, 2014 Nov 18.
Article
in En
| MEDLINE
| ID: mdl-25267651
ABSTRACT
An elusive goal of cellular immune vaccines is the generation of large numbers of antigen-specific T cells in response to subunit immunization. A broad spectrum of cytokines and cell-surface costimulatory molecules are known to shape the programming, magnitude, and repertoire of T cells responding to vaccination. We show here that the majority of innate immune receptor agonist-based vaccine adjuvants unexpectedly depend on IL-27 for eliciting CD4(+) and CD8(+) T-cell responses. This is in sharp contrast to infectious challenge, which generates T-cell responses that are IL-27-independent. Mixed bone marrow chimera experiments demonstrate that IL-27 dependency is T cell-intrinsic, requiring T-cell expression of IL-27Rα. Further, we show that IL-27 dependency not only dictates the magnitude of vaccine-elicited T-cell responses but also is critical for the programming and persistence of high-affinity T cells to subunit immunization. Collectively, our data highlight the unexpected central importance of IL-27 in the generation of robust, high-affinity cellular immune responses to subunit immunization.
Full text:
1
Database:
MEDLINE
Main subject:
Bacterial Vaccines
/
T-Lymphocyte Subsets
/
Interleukins
/
Vaccination
/
Adaptive Immunity
Limits:
Animals
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2014
Type:
Article