Examining platelet adhesion via Stokes flow simulations and microfluidic experiments.
Soft Matter
; 11(2): 355-67, 2015 Jan 14.
Article
in En
| MEDLINE
| ID: mdl-25382632
ABSTRACT
While critically important, the platelet function at the high shear rates typical of the microcirculation is relatively poorly understood. Using a large scale Stokes flow simulation, Zhao et al. recently showed that RBC-induced velocity fluctuations cause platelets to marginate into the RBC free near-wall region [Zhao et al., Physics of Fluids, 2012, 24, 011902]. We extend their work by investigating the dynamics of platelets in shear after margination. An overall platelet adhesion model is proposed in terms of a continuous time Markov process and the transition rates are established with numerical simulations involving platelet-wall adhesion. Hydrodynamic drag and Brownian forces are calculated with the boundary element method, while the RBC collisions are incorporated through an autoregressive process. Hookean springs with first order bond kinetics are used to model receptor-ligand bonds formed between the platelet and the wall. The simulations are compared with in vitro microfluidic experiments involving platelet adhesion to Von Willebrand Factor (VWF) coated surfaces.
Full text:
1
Database:
MEDLINE
Main subject:
Blood Platelets
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Soft Matter
Year:
2015
Type:
Article
Affiliation country:
United States