Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.
Chemistry
; 21(46): 16359-63, 2015 Nov 09.
Article
in En
| MEDLINE
| ID: mdl-26420633
ABSTRACT
A new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements 1)â
detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2)â
universal reporters conjugated with a single fluorophore; and 3)â
fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Salmonella
/
Nucleic Acids
/
Biosensing Techniques
/
Communicable Diseases
/
Nucleic Acid Amplification Techniques
/
DNA-Directed DNA Polymerase
/
Fluorescent Dyes
Type of study:
Diagnostic_studies
/
Health_economic_evaluation
Language:
En
Journal:
Chemistry
Journal subject:
QUIMICA
Year:
2015
Type:
Article