Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants.

Marty-Roix, Robyn; Vladimer, Gregory I; Pouliot, Kimberly; Weng, Dan; Buglione-Corbett, Rachel; West, Kim; MacMicking, John D; Chee, Jonathan D; Wang, Shixia; Lu, Shan; Lien, Egil

Marty-Roix, Robyn; Vladimer, Gregory I; Pouliot, Kimberly; Weng, Dan; Buglione-Corbett, Rachel; West, Kim; MacMicking, John D; Chee, Jonathan D; Wang, Shixia; Lu, Shan; Lien, Egil.

Affiliation

Marty-Roix R; From the Program in Innate Immunity, Division of Infectious Diseases and Immunology and.
Vladimer GI; From the Program in Innate Immunity, Division of Infectious Diseases and Immunology and.
Pouliot K; From the Program in Innate Immunity, Division of Infectious Diseases and Immunology and.
Weng D; From the Program in Innate Immunity, Division of Infectious Diseases and Immunology and.
Buglione-Corbett R; the Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School Worcester, Massachusetts 01605.
West K; the Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School Worcester, Massachusetts 01605.
MacMicking JD; the Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, and.
Chee JD; the Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06510, and.
Wang S; the Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School Worcester, Massachusetts 01605.
Lu S; the Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School Worcester, Massachusetts 01605.
Lien E; From the Program in Innate Immunity, Division of Infectious Diseases and Immunology and the Centre of Molecular Inflammation Research, Department of Cancer and Molecular Medicine, NTNU, 7491 Trondheim, Norway egil.lien@umassmed.edu.

J Biol Chem ; 291(3): 1123-36, 2016 Jan 15.

Article in En | MEDLINE | ID: mdl-26555265

Subject(s)

Adjuvants, Immunologic/pharmacology; Carrier Proteins/metabolism; Dendritic Cells/drug effects; Immunity, Innate/drug effects; Inflammasomes/drug effects; Macrophages/drug effects; Saponins/pharmacology; AIDS Vaccines/agonists; AIDS Vaccines/immunology; Adjuvants, Immunologic/analysis; Adjuvants, Immunologic/chemistry; Animals; Bone Marrow Cells/cytology; Bone Marrow Cells/drug effects; Bone Marrow Cells/immunology; Carrier Proteins/genetics; Cell Survival/drug effects; Cells, Cultured; Dendritic Cells/cytology; Dendritic Cells/immunology; GTP-Binding Proteins/genetics; GTP-Binding Proteins/metabolism; HIV Envelope Protein gp120/agonists; HIV Envelope Protein gp120/immunology; Immunoglobulin G/analysis; Immunoglobulin G/biosynthesis; Inflammasomes/immunology; Inflammasomes/metabolism; Lipid A/agonists; Lipid A/analogs & derivatives; Lipid A/pharmacology; Macrophages/cytology; Macrophages/immunology; Mice, Inbred C57BL; Mice, Knockout; NLR Family, Pyrin Domain-Containing 3 Protein; Saponins/analysis; Saponins/chemistry; Solubility; Th1 Cells/drug effects; Th1 Cells/immunology; Th1 Cells/metabolism; Th2 Cells/drug effects; Th2 Cells/immunology; Th2 Cells/metabolism

Key words

NLRP3; Toll-like receptor 4 (TLR4); adjuvants*; caspase 1 (CASP1); human immunodeficiency virus (HIV); inflammasome; monophosphoryl lipid A; saponin; vaccine

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Full text: 1 Database: MEDLINE Main subject: Saponins / Dendritic Cells / Carrier Proteins / Adjuvants, Immunologic / Inflammasomes / Immunity, Innate / Macrophages Type of study: Diagnostic_studies Language: En Journal: J Biol Chem Year: 2016 Type: Article

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