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Biological changes of Enterococcus faecalis in the viable but nonculturable state.
E, J; Jiang, Y T; Yan, P F; Liang, J P.
Affiliation
  • E J; Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  • Jiang YT; Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  • Yan PF; Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  • Liang JP; Department of Endodontics and Operative Dentistry, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Genet Mol Res ; 14(4): 14790-801, 2015 Nov 23.
Article in En | MEDLINE | ID: mdl-26600540
Enterococcus faecalis may enter a viable but nonculturable (VBNC) state under adverse conditions. E. faecalis, the major bacterial species present in failed root canal treatments, is thought to survive after endodontic treatment by entering a VBNC state. In this study, we characterized the VBNC state of E. faecalis. We designed 3 different protocols to successfully induce the VBNC state. Approximately one-third of bacteria entered a VBNC state after 15-30 days, and all remained viable for at least 2 months. The morphology, glycometabolism, and adhesion capabilities of VBNC cells differed from those of E. faecalis during the exponential growth phase. Specifically, VBNC E. faecalis cells could not decompose lactose, D-mannitol, or D-sorbitol, although they were able to metabolize sucrose. Transmission electron microscopy showed that the morphology of the VBNC E. faecalis cells changed significantly; the cytoplasmic matrix was unevenly condensed and the overall morphology of the cells became irregular, but the cell membranes remained intact. Although the adhesion ability of the bacteria decreased, VBNC E. faecalis could still adhere to collagen fiber type I and tooth dentine. The persistence of this adhesion ability may be important in the virulence of VBNC E. faecalis.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Bacterial Adhesion / Enterococcus faecalis / Dental Pulp Cavity Type of study: Guideline Limits: Humans Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Bacterial Adhesion / Enterococcus faecalis / Dental Pulp Cavity Type of study: Guideline Limits: Humans Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Type: Article Affiliation country: China