Mammalian cells exposed to ionizing radiation: Structural and biochemical aspects.
Appl Radiat Isot
; 108: 12-15, 2016 Feb.
Article
in En
| MEDLINE
| ID: mdl-26656429
ABSTRACT
Acute or chronic exposure to ionizing radiation is a factor that may be hazardous to health. It has been reported that exposure to low doses of radiation (less than 50 mSv/year) and subsequently exposure to high doses produces greater effects in people. It has been reported that people who have been exposed to low doses of radiation (less than 50 mSv/year) and subsequently are exposed to high doses, have greater effects. However, at a molecular and biochemical level, it is an unknown alteration. This study, analyzes the susceptibility of a biological system (HeLa ATCC CCL-2 human cervix cancer cell line) to ionizing radiation (6 and 60 mSv/90 s). Our research considers multiple variables such as total protein profile, mitochondrial metabolic activity (XTT assay), cell viability (Trypan blue exclusion assay), cytoskeleton (actin microfilaments), nuclei (DAPI), and genomic DNA. The results indicate, that cells exposed to ionizing radiation show structural alterations in nuclear phenotype and aneuploidy, further disruption in the tight junctions and consequently on the distribution of actin microfilaments. Similar alterations were observed in cells treated with a genotoxic agent (200 µM H2O2/1h). In conclusion, this multi-criteria assessment enables precise comparisons of the effects of radiation between various line cells. However, it is necessary to determine stress markers for integration of the effects of ionizing radiation.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Cytoskeleton
/
DNA Damage
/
Cell Nucleus
/
Cell Size
/
Dose-Response Relationship, Radiation
/
Mitochondria
Limits:
Humans
Language:
En
Journal:
Appl Radiat Isot
Journal subject:
MEDICINA NUCLEAR
/
SAUDE AMBIENTAL
Year:
2016
Type:
Article