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Proteomic Analysis of the Mammalian Katanin Family of Microtubule-severing Enzymes Defines Katanin p80 subunit B-like 1 (KATNBL1) as a Regulator of Mammalian Katanin Microtubule-severing.
Cheung, Keith; Senese, Silvia; Kuang, Jiaen; Bui, Ngoc; Ongpipattanakul, Chayanid; Gholkar, Ankur; Cohn, Whitaker; Capri, Joseph; Whitelegge, Julian P; Torres, Jorge Z.
Affiliation
  • Cheung K; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
  • Senese S; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
  • Kuang J; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
  • Bui N; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
  • Ongpipattanakul C; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
  • Gholkar A; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095;
  • Cohn W; §Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California 90095;
  • Capri J; §Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California 90095;
  • Whitelegge JP; §Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California 90095; ¶Molecular Biology Institute, University of California, Los Angeles, California, 90095; ‖Jonsson
  • Torres JZ; From the ‡Department of Chemistry and Biochemistry, University of California, Los Angeles, California, 90095; ¶Molecular Biology Institute, University of California, Los Angeles, California, 90095; ‖Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, 90095 torre
Mol Cell Proteomics ; 15(5): 1658-69, 2016 05.
Article in En | MEDLINE | ID: mdl-26929214
The Katanin family of microtubule-severing enzymes is critical for remodeling microtubule-based structures that influence cell division, motility, morphogenesis and signaling. Katanin is composed of a catalytic p60 subunit (A subunit, KATNA1) and a regulatory p80 subunit (B subunit, KATNB1). The mammalian genome also encodes two additional A-like subunits (KATNAL1 and KATNAL2) and one additional B-like subunit (KATNBL1) that have remained poorly characterized. To better understand the factors and mechanisms controlling mammalian microtubule-severing, we have taken a mass proteomic approach to define the protein interaction module for each mammalian Katanin subunit and to generate the mammalian Katanin family interaction network (Katan-ome). Further, we have analyzed the function of the KATNBL1 subunit and determined that it associates with KATNA1 and KATNAL1, it localizes to the spindle poles only during mitosis and it regulates Katanin A subunit microtubule-severing activity in vitro Interestingly, during interphase, KATNBL1 is sequestered in the nucleus through an N-terminal nuclear localization signal. Finally KATNB1 was able to compete the interaction of KATNBL1 with KATNA1 and KATNAL1. These data indicate that KATNBL1 functions as a regulator of Katanin A subunit microtubule-severing activity during mitosis and that it likely coordinates with KATNB1 to perform this function.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Adenosine Triphosphatases / Proteomics / Microtubules Limits: Humans Language: En Journal: Mol Cell Proteomics Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2016 Type: Article

Full text: 1 Database: MEDLINE Main subject: Adenosine Triphosphatases / Proteomics / Microtubules Limits: Humans Language: En Journal: Mol Cell Proteomics Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2016 Type: Article