Abacavir has no prothrombotic effect on platelets in vitro.
J Antimicrob Chemother
; 71(12): 3506-3509, 2016 12.
Article
in En
| MEDLINE
| ID: mdl-27516475
ABSTRACT
OBJECTIVES:
HIV patients exposed to abacavir have an increased risk of myocardial infarction, with contradictory results in the literature. The aim of our study was to determine whether abacavir has a direct effect on platelet activation and aggregation using platelets from healthy donors and from HIV-infected patients under therapy with an undetectable viral load.METHODS:
Platelet-rich plasma (PRP) or whole blood from healthy donors was treated with abacavir (5 or 10 µg/mL) or its active metabolite carbovir diphosphate. Experiments were also performed using blood of HIV-infected patients (nâ=â10) with an undetectable viral load. Platelet aggregation was performed on PRP by turbidimetry and under high shear conditions at 4000 s-1. Platelet procoagulant potential was analysed by measuring thrombin generation by thrombinography.RESULTS:
Abacavir and carbovir diphosphate significantly increased the aggregation of platelets from healthy donors induced by collagen at 2 µg/mL (Pâ=â0.002), but not at 0.5 µg/mL. No effect of abacavir or carbovir diphosphate was observed on platelet aggregation induced by other physiological agonists or by high shear stress, or on thrombin generation. Pretreatment of blood from HIV-infected patients with abacavir produced similar results.CONCLUSIONS:
Our results suggest that abacavir does not significantly influence platelet activation in vitro when incubated with platelets from healthy donors or from HIV-infected patients. It is, however, not excluded that a synergistic effect with other drugs could promote platelet activation and thereby play a role in the pathogenesis of myocardial infarction.
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Database:
MEDLINE
Main subject:
Blood Platelets
/
Dideoxynucleosides
/
Platelet Activation
/
Platelet Aggregation
/
Reverse Transcriptase Inhibitors
/
Anti-HIV Agents
Limits:
Adult
/
Humans
/
Middle aged
Language:
En
Journal:
J Antimicrob Chemother
Year:
2016
Type:
Article
Affiliation country:
France