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[Effects and mechanism of testosterone on the production of inflammatory cytokines and glucose uptake in co-culture of RAW264.7 macrophage and 3T3-L1 adipocytes].
Su, C L; Chen, M; Zhang, P N; Xu, W; Lin, J F.
Affiliation
  • Su CL; Obstetrics and Gynecology Hospital Affiliated to Fudan University, Shanghai 200011, China.
Zhonghua Yi Xue Za Zhi ; 96(33): 2665-2670, 2016 Sep 06.
Article in Zh | MEDLINE | ID: mdl-27666890
Objective: To investigate the effects of testosterone (T) on inflammatory cytokines (IL-6, MCP-1) production, insulin sensitivity of adipocyte and changes of macrophage phenotypes in indirect co-culture of RAW264.7 macrophages and 3T3-L1 adipocytes. Methods: 3T3-L1 preadipocytes were induced to mature in Transwell lower chamber, and then co-cultured with RAW264.7 macrophages in the upper chambers for 72 hours. Testosterone 10 µmol/L was added into indirect co-culture for 24 h. ELISA was used to testing IL-6, MCP-1 concentrations in supernatant. Western blot was used to detecting the phosphorylation of NF kappa B, ERK1/2, and theexpression of CD16/32 and CD206. Glucose transport was assessed by[3H]2-deoxy glucose uptake in adipocytes. Results: Testosterone enhanced inflammatory cytokines (IL-6, MCP-1) production in indirect co-culture of 3T3-L1 adipocytes and RAW264.7 macrophages, promoted the activation of ERK1/2 and nuclear factor kappa B p65, and inhibited glucose uptake in adipocytes. Testosterone facilitated the production of pro-inflammatory M1 macrophages. The above effects of testosterone can be completely reversed by PDTC, and can be partly reversed by PD98059 (70%-90%). Conclusion: NF kappa B and ERK1/2 could be the key proteins for testosterone to promote the production of inflammatory factors, to lead to insulin resistance, and to make macrophages differentiate to pro-inflammatory phenotypes in co-culture of RAW264.7 macrophages and 3T3-L1 adipocytes.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Adipocytes / Macrophages Limits: Animals Language: Zh Journal: Zhonghua Yi Xue Za Zhi Year: 2016 Type: Article Affiliation country: China

Full text: 1 Database: MEDLINE Main subject: Adipocytes / Macrophages Limits: Animals Language: Zh Journal: Zhonghua Yi Xue Za Zhi Year: 2016 Type: Article Affiliation country: China