Establishing a high yielding streptomyces-based cell-free protein synthesis system.

Li, Jian; Wang, He; Kwon, Yong-Chan; Jewett, Michael C

Li, Jian; Wang, He; Kwon, Yong-Chan; Jewett, Michael C.

Affiliation

Li J; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208.
Wang H; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208.
Kwon YC; Masters in Biotechnology Program, Northwestern University, Evanston, Illinois.
Jewett MC; Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208.

Biotechnol Bioeng ; 114(6): 1343-1353, 2017 06.

Article in En | MEDLINE | ID: mdl-28112394

Subject(s)

Biosynthetic Pathways/genetics; Genetic Enhancement/methods; Protein Biosynthesis/physiology; Recombinant Proteins/biosynthesis; Streptomyces/genetics; Streptomyces/metabolism; Artificial Cells/metabolism; Recombinant Proteins/metabolism; Streptomyces/classification; Subcellular Fractions/metabolism

Key words

Streptomyces; cell-free protein synthesis; high GC-content genes; in vitro transcription and translation; natural products; synthetic biology

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Full text: 1 Database: MEDLINE Main subject: Streptomyces / Protein Biosynthesis / Recombinant Proteins / Genetic Enhancement / Biosynthetic Pathways Language: En Journal: Biotechnol Bioeng Year: 2017 Type: Article

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