Quantitative Assay of Macroautophagy Using Pho8â³60 Assay and GFP-Cleavage Assay in Yeast.

Araki, Y; Kira, S; Noda, T

Quantitative Assay of Macroautophagy Using Pho8â³60 Assay and GFP-Cleavage Assay in Yeast.

Araki, Y; Kira, S; Noda, T.

Affiliation

Araki Y; Center for Frontier Oral Science, Graduate School of Dentistry, Osaka University, Osaka, Japan.
Kira S; Center for Frontier Oral Science, Graduate School of Dentistry, Osaka University, Osaka, Japan.
Noda T; Center for Frontier Oral Science, Graduate School of Dentistry, Osaka University, Osaka, Japan; Graduate School of Frontier BioSciences, Osaka University, Osaka, Japan. Electronic address: takenoda@dent.osaka-u.ac.jp.

Methods Enzymol ; 588: 307-321, 2017.

Article in En | MEDLINE | ID: mdl-28237107

Subject(s)

Alkaline Phosphatase/metabolism; Autophagy-Related Protein 8 Family/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae/cytology; Alkaline Phosphatase/genetics; Autophagy; Autophagy-Related Protein 8 Family/genetics; Enzyme Assays/methods; Gene Expression; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/genetics; Vacuoles/genetics; Vacuoles/metabolism

Key words

Alkaline phosphatase; GFP-Atg8; Nonselective autophagy; Pho8â³60; Vacuole; Yeast

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Full text: 1 Database: MEDLINE Main subject: Saccharomyces cerevisiae / Saccharomyces cerevisiae Proteins / Alkaline Phosphatase / Autophagy-Related Protein 8 Family Language: En Journal: Methods Enzymol Year: 2017 Type: Article Affiliation country: Japan

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