Genome-wide analysis of chromatin structures in Trypanosoma brucei using high-resolution MNase-ChIP-seq.
Exp Parasitol
; 180: 2-12, 2017 Sep.
Article
in En
| MEDLINE
| ID: mdl-28286326
ABSTRACT
Specific DNA-protein interactions are the basis for many important cellular mechanisms like the regulation of gene expression or replication. Knowledge about the precise genomic locations of DNA-protein interactions is important because it provides insight into the regulation of these processes. Recently, we have adapted an approach that combines micrococcal nuclease (MNase) digestion of chromatin with chromatin immunoprecipitation in Trypanosoma brucei. Here, we describe in detail how this method can be used to map the genome-wide distribution of nucleosomes or other DNA-binding proteins at high resolution in T. brucei.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Trypanosoma brucei brucei
/
Chromatin
/
Chromatin Immunoprecipitation
/
Micrococcal Nuclease
Language:
En
Journal:
Exp Parasitol
Year:
2017
Type:
Article
Affiliation country:
Germany