An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.

Batiuk, Mykhailo Y; de Vin, Filip; Duqué, Sandra I; Li, Chen; Saito, Takashi; Saido, Takaomi; Fiers, Mark; Belgard, T Grant; Holt, Matthew G

Batiuk, Mykhailo Y; de Vin, Filip; Duqué, Sandra I; Li, Chen; Saito, Takashi; Saido, Takaomi; Fiers, Mark; Belgard, T Grant; Holt, Matthew G.

Affiliation

Batiuk MY; From the Laboratory of Glia Biology, VIB-KU Leuven Center for Brain and Disease Research.
de Vin F; the Laboratory of Glia Biology, KU Leuven Department of Neuroscience, and.
Duqué SI; From the Laboratory of Glia Biology, VIB-KU Leuven Center for Brain and Disease Research.
Li C; the Laboratory of Glia Biology, KU Leuven Department of Neuroscience, and.
Saito T; From the Laboratory of Glia Biology, VIB-KU Leuven Center for Brain and Disease Research.
Saido T; the Laboratory of Glia Biology, KU Leuven Department of Neuroscience, and.
Fiers M; From the Laboratory of Glia Biology, VIB-KU Leuven Center for Brain and Disease Research.
Belgard TG; the Laboratory of Glia Biology, KU Leuven Department of Neuroscience, and.
Holt MG; the Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, Wako-shi, Saitama 351-0106, Japan, and.

J Biol Chem ; 292(21): 8874-8891, 2017 05 26.

Article in En | MEDLINE | ID: mdl-28373281

Subject(s)

Adenosine Triphosphatases; Antibodies/chemistry; Astrocytes/metabolism; Brain Injuries/metabolism; Brain/metabolism; Cation Transport Proteins; Cell Adhesion Molecules, Neuronal; Epitopes; Gene Expression Regulation; Adenosine Triphosphatases/biosynthesis; Adenosine Triphosphatases/chemistry; Animals; Astrocytes/pathology; Brain/pathology; Brain Injuries/pathology; Cation Transport Proteins/biosynthesis; Cation Transport Proteins/chemistry; Cell Adhesion Molecules, Neuronal/biosynthesis; Cell Adhesion Molecules, Neuronal/chemistry; Disease Models, Animal; Epitopes/biosynthesis; Epitopes/chemistry; Female; Male; Mice

Key words

astrocyte; cell surface protein; flow cytometry; immunoblotting; immunohistochemistry; immunoisolation; membrane protein; neuroscience; plasma membrane; single-cell RNA-seq

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Full text: 1 Database: MEDLINE Main subject: Brain / Brain Injuries / Cell Adhesion Molecules, Neuronal / Gene Expression Regulation / Astrocytes / Adenosine Triphosphatases / Cation Transport Proteins / Antibodies / Epitopes Limits: Animals Language: En Journal: J Biol Chem Year: 2017 Type: Article

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