Ca<sup>2+</sup> release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.

Kelu, Jeffrey J; Webb, Sarah E; Parrington, John; Galione, Antony; Miller, Andrew L

Ca²⁺ release via two-pore channel type 2 (TPC2) is required for slow muscle cell myofibrillogenesis and myotomal patterning in intact zebrafish embryos.

Kelu, Jeffrey J; Webb, Sarah E; Parrington, John; Galione, Antony; Miller, Andrew L.

Affiliation

Kelu JJ; Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, PR China.
Webb SE; Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, PR China.
Parrington J; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK.
Galione A; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, UK.
Miller AL; Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, PR China; Marine Biological Laboratory, Woods Hole, MA, USA. Electronic address: almiller@ust.hk.

Dev Biol ; 425(2): 109-129, 2017 05 15.

Article in En | MEDLINE | ID: mdl-28390800

Subject(s)

Body Patterning; Calcium Channels/metabolism; Calcium/metabolism; Embryo, Nonmammalian/metabolism; Kinesins/metabolism; Muscle Development; Muscle Fibers, Slow-Twitch/metabolism; Zebrafish Proteins/metabolism; Zebrafish/embryology; Zebrafish/metabolism; Animals; Base Sequence; Behavior, Animal/drug effects; Body Patterning/drug effects; CRISPR-Cas Systems/genetics; Caffeine/pharmacology; Calcium Signaling/drug effects; Cell Death/drug effects; Cells, Cultured; Embryo, Nonmammalian/drug effects; Gene Knockdown Techniques; Gene Knockout Techniques; Inositol 1,4,5-Trisphosphate Receptors/metabolism; Macrolides/pharmacology; Models, Biological; Morpholinos/pharmacology; Motor Activity/drug effects; Muscle Cells/cytology; Muscle Cells/drug effects; Muscle Cells/metabolism; Muscle Development/drug effects; Muscle Fibers, Slow-Twitch/cytology; Muscle Fibers, Slow-Twitch/drug effects; Phenotype; RNA, Messenger/genetics; RNA, Messenger/metabolism; Ryanodine Receptor Calcium Release Channel/metabolism; Sarcomeres/drug effects; Sarcomeres/metabolism

Key words

CRISPR/Cas9; Ca(2+) signaling; Morpholino oligonucleotides; Myofibrillogenesis; STED super-resolution microscopy; Slow skeletal muscle cell differentiation; Two-pore channel 2; Zebrafish

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Full text: 1 Database: MEDLINE Main subject: Zebrafish / Calcium Channels / Calcium / Kinesins / Muscle Fibers, Slow-Twitch / Body Patterning / Zebrafish Proteins / Muscle Development / Embryo, Nonmammalian Type of study: Prognostic_studies Limits: Animals Language: En Journal: Dev Biol Year: 2017 Type: Article

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