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Kidney triglyceride accumulation in the fasted mouse is dependent upon serum free fatty acids.
Scerbo, Diego; Son, Ni-Huiping; Sirwi, Alaa; Zeng, Lixia; Sas, Kelli M; Cifarelli, Vincenza; Schoiswohl, Gabriele; Huggins, Lesley-Ann; Gumaste, Namrata; Hu, Yunying; Pennathur, Subramaniam; Abumrad, Nada A; Kershaw, Erin E; Hussain, M Mahmood; Susztak, Katalin; Goldberg, Ira J.
Affiliation
  • Scerbo D; Division of Endocrinology, Diabetes, and Metabolism, New York University School of Medicine, New York, NY.
  • Son NH; Institute of Human Nutrition, Columbia University, New York, NY.
  • Sirwi A; Division of Endocrinology, Diabetes, and Metabolism, New York University School of Medicine, New York, NY.
  • Zeng L; Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY.
  • Sas KM; Division of Nephrology, University of Michigan, Ann Arbor, MI.
  • Cifarelli V; Division of Nephrology, University of Michigan, Ann Arbor, MI.
  • Schoiswohl G; Department of Medicine, Washington University, St. Louis, MO.
  • Huggins LA; Division of Endocrinology, University of Pittsburgh, Pittsburgh, PA.
  • Gumaste N; Institute of Molecular Biosciences, University of Graz, Graz, Austria.
  • Hu Y; Division of Endocrinology, Diabetes, and Metabolism, New York University School of Medicine, New York, NY.
  • Pennathur S; Division of Endocrinology, Diabetes, and Metabolism, New York University School of Medicine, New York, NY.
  • Abumrad NA; Division of Endocrinology, Diabetes, and Metabolism, New York University School of Medicine, New York, NY.
  • Kershaw EE; Division of Nephrology, University of Michigan, Ann Arbor, MI.
  • Hussain MM; Department of Medicine, Washington University, St. Louis, MO.
  • Susztak K; Division of Endocrinology, University of Pittsburgh, Pittsburgh, PA.
  • Goldberg IJ; Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, NY.
J Lipid Res ; 58(6): 1132-1142, 2017 06.
Article in En | MEDLINE | ID: mdl-28404638
ABSTRACT
Lipid accumulation is a pathological feature of every type of kidney injury. Despite this striking histological feature, physiological accumulation of lipids in the kidney is poorly understood. We studied whether the accumulation of lipids in the fasted kidney are derived from lipoproteins or NEFAs. With overnight fasting, kidneys accumulated triglyceride, but had reduced levels of ceramide and glycosphingolipid species. Fasting led to a nearly 5-fold increase in kidney uptake of plasma [14C]oleic acid. Increasing circulating NEFAs using a ß adrenergic receptor agonist caused a 15-fold greater accumulation of lipid in the kidney, while mice with reduced NEFAs due to adipose tissue deficiency of adipose triglyceride lipase had reduced triglycerides. Cluster of differentiation (Cd)36 mRNA increased 2-fold, and angiopoietin-like 4 (Angptl4), an LPL inhibitor, increased 10-fold. Fasting-induced kidney lipid accumulation was not affected by inhibition of LPL with poloxamer 407 or by use of mice with induced genetic LPL deletion. Despite the increase in CD36 expression with fasting, genetic loss of CD36 did not alter fatty acid uptake or triglyceride accumulation. Our data demonstrate that fasting-induced triglyceride accumulation in the kidney correlates with the plasma concentrations of NEFAs, but is not due to uptake of lipoprotein lipids and does not involve the fatty acid transporter, CD36.
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Full text: 1 Database: MEDLINE Main subject: Triglycerides / Fasting / Fatty Acids, Nonesterified / Kidney Limits: Animals Language: En Journal: J Lipid Res Year: 2017 Type: Article

Full text: 1 Database: MEDLINE Main subject: Triglycerides / Fasting / Fatty Acids, Nonesterified / Kidney Limits: Animals Language: En Journal: J Lipid Res Year: 2017 Type: Article