Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications.
Pediatr Blood Cancer
; 64(12)2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-28598542
ABSTRACT
BACKGROUND:
Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs. PROCEDURE We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines.RESULTS:
Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (â¼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells.CONCLUSIONS:
Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Cerebellar Neoplasms
/
Minichromosome Maintenance Proteins
/
Medulloblastoma
Limits:
Humans
Language:
En
Journal:
Pediatr Blood Cancer
Journal subject:
HEMATOLOGIA
/
NEOPLASIAS
/
PEDIATRIA
Year:
2017
Type:
Article
Affiliation country:
Austria