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Constitutive expression of human gastric lipase in Pichia pastoris and site-directed mutagenesis of key lid-stabilizing residues.
Sams, Laura; Amara, Sawsan; Chakroun, Almahdi; Coudre, Sébastien; Paume, Julie; Giallo, Jacqueline; Carrière, Frédéric.
Affiliation
  • Sams L; CNRS, Aix Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France; GERME S.A., Technopôle Marseille Provence Château-Gombert, ZAC la Baronne, 12 Rue Marc Donadille, 13013 Marseille, France.
  • Amara S; CNRS, Aix Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France; Lipolytech, Zone Luminy Biotech, 163 avenue de Luminy, 13288 Marseille Cedex 09, France.
  • Chakroun A; CNRS, Aix Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
  • Coudre S; CNRS, Aix Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
  • Paume J; GERME S.A., Technopôle Marseille Provence Château-Gombert, ZAC la Baronne, 12 Rue Marc Donadille, 13013 Marseille, France.
  • Giallo J; GERME S.A., Technopôle Marseille Provence Château-Gombert, ZAC la Baronne, 12 Rue Marc Donadille, 13013 Marseille, France.
  • Carrière F; CNRS, Aix Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse UMR7282, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Electronic address: carriere@imm.cnrs.fr.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1862(10 Pt A): 1025-1034, 2017 Oct.
Article in En | MEDLINE | ID: mdl-28694218
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Full text: 1 Database: MEDLINE Main subject: Pichia / Gene Expression / Lipase Limits: Humans Language: En Journal: Biochim Biophys Acta Mol Cell Biol Lipids Year: 2017 Type: Article Affiliation country: France
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Full text: 1 Database: MEDLINE Main subject: Pichia / Gene Expression / Lipase Limits: Humans Language: En Journal: Biochim Biophys Acta Mol Cell Biol Lipids Year: 2017 Type: Article Affiliation country: France