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Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures.
Tsunematsu, Hiroto; Uyeda, Akiko; Yamamoto, Nobuhiko; Sugo, Noriyuki.
Affiliation
  • Tsunematsu H; Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Uyeda A; Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Yamamoto N; Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Sugo N; Cellular and Molecular Neurobiology, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan. sugo@fbs.osaka-u.ac.jp.
BMC Neurosci ; 18(1): 55, 2017 08 01.
Article in En | MEDLINE | ID: mdl-28764650
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Full text: 1 Database: MEDLINE Main subject: Immunohistochemistry / CRISPR-Cas Systems / Microscopy, Fluorescence / Neurons Type of study: Prognostic_studies Limits: Animals Language: En Journal: BMC Neurosci Journal subject: NEUROLOGIA Year: 2017 Type: Article Affiliation country: Japan
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Full text: 1 Database: MEDLINE Main subject: Immunohistochemistry / CRISPR-Cas Systems / Microscopy, Fluorescence / Neurons Type of study: Prognostic_studies Limits: Animals Language: En Journal: BMC Neurosci Journal subject: NEUROLOGIA Year: 2017 Type: Article Affiliation country: Japan