A novel design of HA-coated nanoparticles co-encapsulating plasmid METase and 5-Fu shows enhanced application in targeting gastric cancer stem cells.
Biol Chem
; 399(3): 293-303, 2018 02 23.
Article
in En
| MEDLINE
| ID: mdl-29016350
ABSTRACT
Nanoparticles (NPs) are recognized as an attractive vehicles for cancer treatment due to their targeted drug release. Gastric cancer is an important killer disease, and its therapy methods still need improvement. The NPs were prepared using a precipitation method, and were evaluated using transmission electron microscopy (TEM). MTT and Transwell assays were used to determine cell viability and apoptosis. In vivo experiments were performed to validate the effects of NPs on tumor growth. Methioninase (METase)/5-Fu co-encaspulated NPs showed highest ζ size and lowest ζ potential than other NPs. The migration and tumorsphere formation ability of CD44(+) was stronger than CD44(-). The effects of METase/5-Fu co-encaspulated NPs on inhibition cell growth was stronger than that of 5-Fu encaspulated NPs, while HA coated NPs showed significant target ability than that NPs without HA. METase supplementation promoted the inhibition effect of 5-Fu on thymidylate synthetase (TS), as well as cell apoptosis. The in vivo experiments demonstrated that HA coated NPs significantly inhibited tumor growth. It was concluded that HA-coated NPs enhance the target ability, while METase/5-Fu co-encaspulated NPs promote the inhibition effects on tumor growth in gastric cancer.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
Carbon-Sulfur Lyases
/
Drug Design
/
Nanoparticles
/
Fluorouracil
/
Hyaluronic Acid
/
Antimetabolites, Antineoplastic
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Biol Chem
Journal subject:
BIOQUIMICA
Year:
2018
Type:
Article
Affiliation country:
China