Ethyl lucidenates A reverses P-glycoprotein mediated vincristine resistance in K562/A02 cells.
Nat Prod Res
; 33(5): 732-735, 2019 Mar.
Article
in En
| MEDLINE
| ID: mdl-29130339
ABSTRACT
Multidrug resistance is a major unresolved obstacle to successful cancer chemotherapy. It is often associated with an elevated efflux of a variety of anticancer drugs by ATP-binding cassette transporters including P-glycoprotein, BCRP and MRP1. In this study, the reversal effect of Ethyl lucidenates A on K562/A02 cells was investigated. At concentrations of 10 µM, Ethyl lucidenates A could reverse the resistance of K562/A02 to vincristine up to 7.59 folds. Mechanistically, Ethyl lucidenates A could increase the intracellular accumulation of vincristine in K562/A02 cells through inhibiting the P-glycoprotein mediated drug-transport activity by rhodamine accumulation assay and cell cycle analysis. Further mechanistic investigation found that Ethyl lucidenates A did not alter P-glycoprotein expression. In conclusion, Ethyl lucidenates A could reverse the multidrug resistance of K562/A02 cells via its influence on P-glycoprotein drug-transport activity and thus, be a potential multidrug resistance reversal agent.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Vincristine
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Lanosterol
/
Antineoplastic Agents
Limits:
Humans
Language:
En
Journal:
Nat Prod Res
Year:
2019
Type:
Article
Affiliation country:
China