Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics.
Plant Physiol
; 177(2): 476-489, 2018 06.
Article
in En
| MEDLINE
| ID: mdl-29703867
ABSTRACT
Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.
Full text:
1
Database:
MEDLINE
Main subject:
Plant Growth Regulators
/
Arabidopsis
/
Tandem Mass Spectrometry
/
Metabolomics
Language:
En
Journal:
Plant Physiol
Year:
2018
Type:
Article
Affiliation country:
Czech Republic