A Versatile and Convenient Synthesis of 34 S-Labeled Phosphorothioate Oligonucleotides.
Chembiochem
; 19(19): 2114-2119, 2018 10 04.
Article
in En
| MEDLINE
| ID: mdl-30062829
ABSTRACT
A synthetic protocol for 34 S-labeled phosphorothioate oligonucleotides (PS ONs) was developed to facilitate MS-based assay analysis. This was enabled by a highly efficient, two-step, one-pot synthesis of 34 S-labeled phenylacetyl disulfide (34 S-PADS), starting from 34 S-enriched elemental sulfur (34 S8 ). 34 S-PADS was subsequently used for stable isotope labeling (SIL) of oligonucleotides containing a phosphorothioate backbone. The 34 S-SIL PS ONs are shown to retain the same melting temperature, antisense activity, and secondary structure as those of the corresponding unlabeled 32 S PS ONs.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Phenylacetates
/
Sulfides
/
Oligonucleotides, Antisense
/
Phosphorothioate Oligonucleotides
Limits:
Humans
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2018
Type:
Article
Affiliation country:
Sweden