The replicative histone chaperone CAF1 is essential for the maintenance of identity and genome integrity in adult stem cells.
Development
; 145(17)2018 08 28.
Article
in En
| MEDLINE
| ID: mdl-30093554
ABSTRACT
Chromatin packaging and modifications are important to define the identity of stem cells. How chromatin properties are retained over multiple cycles of stem cell replication, while generating differentiating progeny at the same time, remains a challenging question. The chromatin assembly factor CAF1 is a conserved histone chaperone, which assembles histones H3 and H4 onto newly synthesized DNA during replication and repair. Here, we have investigated the role of CAF1 in the maintenance of germline stem cells (GSCs) in Drosophila ovaries. We depleted P180, the large subunit of CAF1, in germ cells and found that it was required in GSCs to maintain their identity. In the absence of P180, GSCs still harbor stem cell properties but concomitantly express markers of differentiation. In addition, P180-depleted germ cells exhibit elevated levels of DNA damage and de-repression of the transposable I element. These DNA damages activate p53- and Chk2-dependent checkpoints pathways, leading to cell death and female sterility. Altogether, our work demonstrates that chromatin dynamics mediated by CAF1 play an important role in both the regulation of stem cell identity and genome integrity.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Ovary
/
Drosophila Proteins
/
Genomic Instability
/
Drosophila melanogaster
/
Adult Stem Cells
/
Retinoblastoma-Binding Protein 4
Limits:
Animals
Language:
En
Journal:
Development
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2018
Type:
Article
Affiliation country:
France