Development of a multiplex real-time PCR assay using two thermocycling platforms for detection of major bacterial pathogens associated with bovine respiratory disease complex from clinical samples.

Loy, John D; Leger, Laura; Workman, Aspen M; Clawson, Michael L; Bulut, Ece; Wang, Bing

Loy, John D; Leger, Laura; Workman, Aspen M; Clawson, Michael L; Bulut, Ece; Wang, Bing.

Affiliation

Loy JD; School of Veterinary Medicine and Biomedical Sciences (Loy, Leger), University of Nebraska-Lincoln, Lincoln, NE.
Leger L; Department of Food Science and Technology (Bulut, Wang), University of Nebraska-Lincoln, Lincoln, NE.
Workman AM; U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE (Workman, Clawson).
Clawson ML; School of Veterinary Medicine and Biomedical Sciences (Loy, Leger), University of Nebraska-Lincoln, Lincoln, NE.
Bulut E; Department of Food Science and Technology (Bulut, Wang), University of Nebraska-Lincoln, Lincoln, NE.
Wang B; U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, NE (Workman, Clawson).

J Vet Diagn Invest ; 30(6): 837-847, 2018 Nov.

Article in En | MEDLINE | ID: mdl-30239324

Subject(s)

Bovine Respiratory Disease Complex/diagnosis; Multiplex Polymerase Chain Reaction/veterinary; Real-Time Polymerase Chain Reaction/veterinary; Animals; Bovine Respiratory Disease Complex/classification; Bovine Respiratory Disease Complex/microbiology; Cattle; Lung/microbiology; Mannheimia haemolytica/isolation & purification; Multiplex Polymerase Chain Reaction/instrumentation; Multiplex Polymerase Chain Reaction/methods; Mycoplasma Infections/classification; Mycoplasma Infections/diagnosis; Mycoplasma Infections/microbiology; Mycoplasma Infections/veterinary; Mycoplasma bovis/isolation & purification; Nasopharynx/microbiology; Pasteurella multocida/isolation & purification; Pasteurellaceae/isolation & purification; Pasteurellaceae Infections/classification; Pasteurellaceae Infections/diagnosis; Pasteurellaceae Infections/microbiology; Pasteurellaceae Infections/veterinary; Real-Time Polymerase Chain Reaction/instrumentation; Real-Time Polymerase Chain Reaction/methods

Key words

Bacterial pathogens; bovine respiratory disease; clinical specimens; real-time PCR; receiver operating characteristic analysis

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Full text: 1 Database: MEDLINE Main subject: Bovine Respiratory Disease Complex / Multiplex Polymerase Chain Reaction / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies / Evaluation_studies / Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: J Vet Diagn Invest Journal subject: MEDICINA VETERINARIA Year: 2018 Type: Article Affiliation country: Niger

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