Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation.
Methods Mol Biol
; 1874: 179-190, 2019.
Article
in En
| MEDLINE
| ID: mdl-30353514
ABSTRACT
The CRISPR-Cas9 system in bacteria and archaea has recently been exploited for genome editing in various model organisms, including the mice. In this scheme, components of the CRISPR-Cas9 system are delivered into the mouse zygote and mutant mice carrying genetic modifications derived. Although microinjection has been the technology of choice, electroporation has also emerged and been proven to be effective delivering CRISPR-Cas9 reagents into the mouse zygote. Here, we describe the experimental protocol employing electroporation to deliver CRISPR-Cas9 reagents into mouse embryos and derive gene-edited mutant mice.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Zygote
/
Electroporation
/
CRISPR-Cas Systems
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Type:
Article
Affiliation country:
United States