Cav1.3 is upregulated in osteoporosis rat model and promotes osteoclast differentiation from preosteoclast cell line RAW264.7.
J Cell Physiol
; 234(8): 12821-12827, 2019 08.
Article
in En
| MEDLINE
| ID: mdl-30741411
ABSTRACT
BACKGROUND:
Osteoporosis (OP) is a systemic osteopathy with increased bone fragility and increased risk of fracture. Osteoclasts (OC) are the key target cells in the treatment of osteoporosis. We aimed to research the role of L-type calcium channel protein Cav1.3 in OC differentiation in this study.METHODS:
OP rat model was established to detect the expression level of Cav1.3. Tartrate-resistant acid phosphatase assay was used to measure the differentiation of osteoclast during receptor activator of nuclear factor κ-Β ligand (RANKL)-induced osteoclasts formation. The expression of bone differentiation-related proteins were detected by western blot analysis.RESULTS:
Cav1.3 is upregulated in OP rats. Knockdown of Cav1.3 inhibits the differentiation of RAW264.7. Cav1.3 regulates the cell differentiation and bone resorption of RAW264.7 during RANKL-induced osteoclasts formation, which is accompanied by upregulation of CaMK II, p-CERB, AP-1, NFATC1, and NF-κB.CONCLUSION:
Cav1.3 plays an important role in osteoporosis and the differentiation of osteoclast, which might be involved with the bone differentiation-related proteins.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Osteoclasts
/
Osteoporosis
/
Calcium Channels
/
Cell Differentiation
/
Up-Regulation
/
Calcium Channels, L-Type
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Cell Physiol
Year:
2019
Type:
Article
Affiliation country:
China