Epidermal loss of phospholipase Cδ1 attenuates irritant contact dermatitis.
Biochem Biophys Res Commun
; 511(2): 330-335, 2019 04 02.
Article
in En
| MEDLINE
| ID: mdl-30791982
ABSTRACT
Irritant contact dermatitis (ICD) is one of the most common inflammatory skin diseases caused by exposure to chemical irritants. Since chemical irritants primarily damage keratinocytes, these cells play a pivotal role in ICD. One of the phosphoinositide-metabolizing enzymes, phospholipase C (PLC) δ1, is abundantly expressed in keratinocytes. However, the role of PLCδ1 in ICD remains to be clarified. Here, we found that croton oil (CrO)-induced ear swelling, a feature of ICD, was attenuated in keratinocyte-specific PLCδ1 knockout mice (PLCδ1 cKO mice). Dendritic epidermal T cells (DETCs), which have a protective role against ICD, were activated in the epidermis of the PLCδ1 cKO mice. In addition, the skin of CrO-treated PLCδ1 cKO mice showed increased infiltration of Gr1+CD11b+ myeloid cells. Of note, elimination of Gr1+CD11b+ myeloid cells restored CrO-induced ear swelling in PLCδ1 cKO mice to a similar level as that in control mice. Taken together, our results strongly suggest that epidermal loss of PLCδ1 protects mice from ICD through induction of Gr1+CD11b+ myeloid cells and activation of DETCs.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Dermatitis, Contact
/
Phospholipase C delta
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2019
Type:
Article
Affiliation country:
Japan