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Fluorescence lifetime imaging of intracellular magnesium content in live cells.
Sargenti, Azzurra; Candeo, Alessia; Farruggia, Giovanna; D'Andrea, Cosimo; Cappadone, Concettina; Malucelli, Emil; Valentini, Gianluca; Taroni, Paola; Iotti, Stefano.
Affiliation
  • Sargenti A; Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. stefano.iotti@unibo.it.
  • Candeo A; Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20113, Milano, Italy.
  • Farruggia G; Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. stefano.iotti@unibo.it and National Institute of Biostructures and Biosystems (NIBB), Rome, Italy.
  • D'Andrea C; Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20113, Milano, Italy.
  • Cappadone C; Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. stefano.iotti@unibo.it.
  • Malucelli E; Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. stefano.iotti@unibo.it.
  • Valentini G; Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20113, Milano, Italy.
  • Taroni P; Department of Physics, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20113, Milano, Italy.
  • Iotti S; Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. stefano.iotti@unibo.it and National Institute of Biostructures and Biosystems (NIBB), Rome, Italy.
Analyst ; 144(6): 1876-1880, 2019 Mar 21.
Article in En | MEDLINE | ID: mdl-30810548
ABSTRACT
The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.
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Full text: 1 Database: MEDLINE Main subject: Spectrometry, Fluorescence / Bone Neoplasms / Osteosarcoma / Optical Imaging / Magnesium Limits: Humans Language: En Journal: Analyst Year: 2019 Type: Article Affiliation country: Italy
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Full text: 1 Database: MEDLINE Main subject: Spectrometry, Fluorescence / Bone Neoplasms / Osteosarcoma / Optical Imaging / Magnesium Limits: Humans Language: En Journal: Analyst Year: 2019 Type: Article Affiliation country: Italy