Your browser doesn't support javascript.
loading
Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System.
Shum, Eleen Y; Walczak, Elisabeth M; Chang, Christina; Christina Fan, H.
Affiliation
  • Shum EY; BD Biosciences, San Jose, CA, USA. Eleen.Shum@bd.com.
  • Walczak EM; BD Biosciences, San Jose, CA, USA.
  • Chang C; BD Biosciences, San Jose, CA, USA.
  • Christina Fan H; BD Biosciences, San Jose, CA, USA.
Adv Exp Med Biol ; 1129: 63-79, 2019.
Article in En | MEDLINE | ID: mdl-30968361
In this review, we describe the BD Rhapsody™ Single-Cell Analysis System, a platform that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries, including whole transcriptome analysis for discovery biology and targeted RNA analysis for high sensitivity transcript detection. The BD Rhapsody system can be used with emerging applications, such as BD™ AbSeq assays, to profile gene expression in both mRNA and protein level to provide ultra-high resolution analysis of single cells.
Subject(s)
Key words

Full text: 1 Database: MEDLINE Main subject: RNA, Messenger / Gene Expression Profiling / Single-Cell Analysis / High-Throughput Nucleotide Sequencing Language: En Journal: Adv Exp Med Biol Year: 2019 Type: Article Affiliation country: United States

Full text: 1 Database: MEDLINE Main subject: RNA, Messenger / Gene Expression Profiling / Single-Cell Analysis / High-Throughput Nucleotide Sequencing Language: En Journal: Adv Exp Med Biol Year: 2019 Type: Article Affiliation country: United States