Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts.
J Immunol
; 139(7): 2317-24, 1987 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-3116078
ABSTRACT
Human endothelial cells and dermal fibroblasts both expressed a membrane-associated interleukin 1 (IL-1) activity when stimulated with either recombinant tumor necrosis factor (TNF) or recombinant lymphotoxin but stimulated endothelial cells expressed significantly more membrane IL-1 per cell than did fibroblasts. Lipopolysaccharide induced membrane IL-1 activity on endothelial cells but not fibroblasts. Interferon-gamma treatment of endothelial cells and fibroblasts had no direct effect on membrane IL-1 expression and little effect when used as a pretreatment for TNF or lipopolysaccharide stimulation. Endothelial cell membrane IL-1 activity was induced within 24 hr of culture with TNF or lipopolysaccharide, and increased up to 72 hr of incubation. Antibodies raised against human monocyte-derived IL-1 species neutralized the membrane IL-1 activity of TNF-stimulated endothelial cells. Both absorption studies and neutralization with specific sera indicated that endothelial cell membrane IL-1 is structurally related to IL-1 alpha. Endothelial cells expressed both IL-1 beta mRNA in response to TNF, lymphotoxin, and recombinant IL-1 species, as detected by Northern blot analysis. These studies demonstrate that endothelial cells can be activated to express a cell-surface IL-1 activity which is structurally, as well as functionally, related to the secreted form of IL-1.
Search on Google
Database:
MEDLINE
Main subject:
Endothelium, Vascular
/
Lipopolysaccharides
/
Interleukin-1
/
Lymphotoxin-alpha
/
Tumor Necrosis Factor-alpha
/
Fibroblasts
/
Membrane Proteins
Limits:
Humans
Language:
En
Journal:
J Immunol
Year:
1987
Type:
Article