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Molecular basis for interactions between an acyl carrier protein and a ketosynthase.
Milligan, Jacob C; Lee, D John; Jackson, David R; Schaub, Andrew J; Beld, Joris; Barajas, Jesus F; Hale, Joseph J; Luo, Ray; Burkart, Michael D; Tsai, Shiou-Chuan.
Affiliation
  • Milligan JC; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA.
  • Lee DJ; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
  • Jackson DR; Department of Chemistry, University of California, Irvine, Irvine, CA, USA.
  • Schaub AJ; Department of Chemistry, University of California, Irvine, Irvine, CA, USA.
  • Beld J; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
  • Barajas JF; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA.
  • Hale JJ; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
  • Luo R; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA.
  • Burkart MD; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA. mburkart@ucsd.edu.
  • Tsai SC; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA. sctsai@uci.edu.
Nat Chem Biol ; 15(7): 669-671, 2019 07.
Article in En | MEDLINE | ID: mdl-31209348
ABSTRACT
Fatty acid synthases are dynamic ensembles of enzymes that can biosynthesize long hydrocarbon chains efficiently. Here we visualize the interaction between the Escherichia coli acyl carrier protein (AcpP) and ß-ketoacyl-ACP-synthase I (FabB) using X-ray crystallography, NMR, and molecular dynamics simulations. We leveraged this structural information to alter lipid profiles in vivo and provide a molecular basis for how protein-protein interactions can regulate the fatty acid profile in E. coli.
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Full text: 1 Database: MEDLINE Main subject: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Acyl Carrier Protein / Escherichia coli Proteins / Fatty Acid Synthase, Type II Language: En Journal: Nat Chem Biol Journal subject: BIOLOGIA / QUIMICA Year: 2019 Type: Article Affiliation country: United States
Fulltext
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Full text: 1 Database: MEDLINE Main subject: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase / Acyl Carrier Protein / Escherichia coli Proteins / Fatty Acid Synthase, Type II Language: En Journal: Nat Chem Biol Journal subject: BIOLOGIA / QUIMICA Year: 2019 Type: Article Affiliation country: United States