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Cell mediated and innate immune responses in pigs following vaccination and challenge with Toxoplasma parasites.
Bartley, Paul M; Burrells, Alison; Benavides, Julio; Canton, German; Garcia, Joao L; Thomson, Jackie; Chianini, Fransesca; Innes, Elisabeth A; Katzer, Frank.
Affiliation
  • Bartley PM; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK. Electronic address: paul.bartley@moredun.ac.uk.
  • Burrells A; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK.
  • Benavides J; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK; Instituto de Ganadería de Montaña (CSIC-ULE), León, Spain.
  • Canton G; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK; Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Balcarce, Argentina.
  • Garcia JL; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK; Laboratório de Protozoologia, Universidade de Londrina, Brazil.
  • Thomson J; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK.
  • Chianini F; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK.
  • Innes EA; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK.
  • Katzer F; Moredun Research Institute, Pentlands Science Park, Bush Loan, Midlothian, EH26 0PZ, Scotland, UK.
Vet Parasitol ; 275: 108963, 2019 Nov.
Article in En | MEDLINE | ID: mdl-31669836
ABSTRACT
Toxoplasma gondii has a worldwide distribution and can infect almost all warm blooded animals including pigs and humans. This study aims to examine the immune responses induced in pigs following vaccination (live S48 tachyzoites) and/or challenge with T. gondii oocysts, through the examination of changes in levels of transcription in CD4, CD8α, IFN-γ, IL-12p35, CXCR3, MyD88. The experiment involved four groups of animals; pigs in group 1 (Challenged) (Chal) were challenged orally with (1 × 103 oocysts) on day 28 of the experiment. Pigs in group 2 (Vaccinated /Challenged) (Vac/Chal) were vaccinated (S48 isolate tachyzoites) on day 0, then challenged on day 28. The group 3 (Vaccinated) (Vac) animals were vaccinated (S48 isolate tachyzoites) on day 0 of the experiment. Finally the group 4 (control) pigs remained non-vaccinated and non-challenged. All animals were culled 6 weeks post challenge. At post mortem samples of retropharyngeal lymph node (RLN), mesenteric LN (MLN) and spleen were collected, RNA was extracted and cDNA synthesised. The results showed significant increases in IFN-γ expression in samples from groups 1 (Chal) and 2 (Vac/Chal) (RLN) and groups 1, 2 and 3 (Vac) (spleen) and in MyD88 expression (RLN) in samples from groups 1, 2 and 3 compared to the group 4 (control) animals. Significant increases were also observed in CD8α expression in group 1 (Chal) (RLN) and groups 1 and 2 (Vac/Chal) (RLN and MLN) compared against group 4 (control) and group 3 (Vac) respectively. Conversely, significant down regulation of CD4 and/or IL-12p35 transcription was found in at least one sample from groups 1 (Chal), 2 (Vac/Chal) and 3 (Vac) compared to group 4 (control) pigs. This study demonstrates that cell mediated and innate immune responses are generated in pigs following exposure to T. gondii parasites (oocysts or tachyzoites), key amongst them appear to be IFN-γ, MyD88 and CD8α.
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Full text: 1 Database: MEDLINE Main subject: Swine Diseases / Toxoplasmosis, Animal / Immunity, Cellular / Immunity, Innate Limits: Animals Language: En Journal: Vet Parasitol Year: 2019 Type: Article

Full text: 1 Database: MEDLINE Main subject: Swine Diseases / Toxoplasmosis, Animal / Immunity, Cellular / Immunity, Innate Limits: Animals Language: En Journal: Vet Parasitol Year: 2019 Type: Article