Cloning, Purification, and Characterization of the Catalytic C-Terminal Domain of the Human 3-Hydroxy-3-methyl glutaryl-CoA Reductase: An Effective, Fast, and Easy Method for Testing Hypocholesterolemic Compounds.

Curcio, Rosita; Aiello, Donatella; Vozza, Angelo; Muto, Luigina; Martello, Emanuela; Cappello, Anna Rita; Capobianco, Loredana; Fiermonte, Giuseppe; Siciliano, Carlo; Napoli, Anna; Dolce, Vincenza

Curcio, Rosita; Aiello, Donatella; Vozza, Angelo; Muto, Luigina; Martello, Emanuela; Cappello, Anna Rita; Capobianco, Loredana; Fiermonte, Giuseppe; Siciliano, Carlo; Napoli, Anna; Dolce, Vincenza.

Affiliation

Curcio R; Department of Pharmacy, Health, and Nutritional Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy.
Aiello D; Department of Chemistry and Chemical Technologies, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy.
Vozza A; Laboratory of Biochemistry and Molecular Biology, Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, 70125, Bari, Italy.
Muto L; Department of Pharmacy, Health, and Nutritional Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy.
Martello E; Department of Pharmacy, Health, and Nutritional Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy.
Cappello AR; Department of Pharmacy, Health, and Nutritional Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy.
Capobianco L; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100, Lecce, Italy.
Fiermonte G; Laboratory of Biochemistry and Molecular Biology, Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, 70125, Bari, Italy.
Siciliano C; Department of Pharmacy, Health, and Nutritional Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy.
Napoli A; Department of Chemistry and Chemical Technologies, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy. amc.napoli@unical.it.
Dolce V; Department of Pharmacy, Health, and Nutritional Sciences, University of Calabria, 87036, Arcavacata di Rende, Cosenza, Italy. vincenza.dolce@unical.it.

Mol Biotechnol ; 62(2): 119-131, 2020 Feb.

Article in En | MEDLINE | ID: mdl-31758489

Subject(s)

Hydroxymethylglutaryl CoA Reductases/chemistry; Hydroxymethylglutaryl CoA Reductases/genetics; Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology; Amino Acid Sequence/genetics; Catalytic Domain; Chromatography, Affinity; Cloning, Molecular; Drug Evaluation, Preclinical/methods; Enzyme Assays/methods; Escherichia coli/genetics; Gene Expression; Humans; Hydroxymethylglutaryl CoA Reductases/isolation & purification; Hydroxymethylglutaryl CoA Reductases/metabolism; Hydroxymethylglutaryl-CoA Reductase Inhibitors/chemistry; Hydroxymethylglutaryl-CoA Reductase Inhibitors/isolation & purification; Hydroxymethylglutaryl-CoA Reductase Inhibitors/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Tandem Mass Spectrometry

Key words

Affinity chromatography; Bacterial expression; Enzymatic activity; HMGR; MALDI MS and MS/MS; Screening of statin-like molecules

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Full text: 1 Database: MEDLINE Main subject: Hydroxymethylglutaryl-CoA Reductase Inhibitors / Hydroxymethylglutaryl CoA Reductases Limits: Humans Language: En Journal: Mol Biotechnol Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2020 Type: Article Affiliation country: Italy

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