Manufacturing Chimeric Antigen Receptor (CAR) T Cells for Adoptive Immunotherapy.
J Vis Exp
; (154)2019 12 17.
Article
in En
| MEDLINE
| ID: mdl-31904011
ABSTRACT
Adoptive immunotherapy holds promise for the treatment of cancer and infectious disease. We describe a simple approach to transduce primary human T cells with chimeric antigen receptor (CAR) and expand their progeny ex vivo. We include assays to measure CAR expression as well as differentiation, proliferative capacity and cytolytic activity. We describe assays to measure effector cytokine production and inflammatory cytokine secretion in CAR T cells. Our approach provides a reliable and comprehensive method to culture CAR T cells for preclinical models of adoptive immunotherapy.
Full text:
1
Database:
MEDLINE
Main subject:
Receptors, Antigen, T-Cell
/
T-Lymphocytes
/
Immunotherapy, Adoptive
/
Receptors, Chimeric Antigen
Limits:
Animals
/
Humans
Language:
En
Journal:
J Vis Exp
Year:
2019
Type:
Article