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Effect of a novel bioceramic root canal sealer on the angiogenesis-enhancing potential of assorted human odontogenic stem cells compared with principal tricalcium silicate-based cements.
Olcay, Keziban; Tasli, Pakize Neslihan; Güven, Esra Pamukçu; Ülker, Gül Merve Yalçin; Ögüt, Emine Esen; Çiftçioglu, Elif; Kiratli, Binnur; Sahin, Fikrettin.
Affiliation
  • Olcay K; Istanbul Medipol University, Faculty of Dentistry, Department of Endodontics, Istanbul, Turkey.
  • Tasli PN; Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Istanbul, Turkey.
  • Güven EP; Istanbul Okan University, Faculty of Dentistry, Department of Endodontics, Istanbul, Turkey.
  • Ülker GMY; Istanbul Okan University, Faculty of Dentistry. Department of Oral and Maxillofacial Surgery, Istanbul, Turkey.
  • Ögüt EE; Istanbul Medipol University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey.
  • Çiftçioglu E; Istanbul Okan University, Faculty of Dentistry, Department of Endodontics, Istanbul, Turkey.
  • Kiratli B; Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Istanbul, Turkey.
  • Sahin F; Yeditepe University, Faculty of Engineering and Architecture, Department of Genetics and Bioengineering, Istanbul, Turkey.
J Appl Oral Sci ; 28: e20190215, 2020.
Article in En | MEDLINE | ID: mdl-31939521
OBJECTIVE: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). METHODOLOGY: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. RESULTS: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). CONCLUSION: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs.
Subject(s)

Full text: 1 Database: MEDLINE Main subject: Root Canal Filling Materials / Stem Cells / Ceramics / Silicates / Calcium Compounds / Angiogenesis Inducing Agents Type of study: Evaluation_studies Limits: Humans Language: En Journal: J Appl Oral Sci Journal subject: ODONTOLOGIA Year: 2020 Type: Article Affiliation country: Turkey

Full text: 1 Database: MEDLINE Main subject: Root Canal Filling Materials / Stem Cells / Ceramics / Silicates / Calcium Compounds / Angiogenesis Inducing Agents Type of study: Evaluation_studies Limits: Humans Language: En Journal: J Appl Oral Sci Journal subject: ODONTOLOGIA Year: 2020 Type: Article Affiliation country: Turkey