Limitations of cell-lineage-specific non-dynamic gene recombination in CD11c.Cre+ITGA4fl/fl mice.
J Neuroimmunol
; 344: 577245, 2020 07 15.
Article
in En
| MEDLINE
| ID: mdl-32335319
ABSTRACT
BACKGROUND:
The Cre-lox system is a non-dynamic method of gene modification and characterization. Promoters thought to be relatively cell-specific are utilized for generation of cell-lineage-specific gene modifications.METHODS:
CD11c.Cre+ITGA4fl/fl mice were generated to abolish the expression of ITGA (α4-integrin) in CD11c+ cells. Ex vivo flow cytometry studies were used to assess the expression of cellular surface markers in different lymphoid compartments and leukocytes subsets after Cre-mediated recombination.RESULTS:
A significant reduction of α4-integrin expression among CD11c+- cells was achieved in CD11c.Cre+ITGA4fl/fl mice in primary and secondary lymphoid tissues. A similar reduction in the expression of α4-integrin was also observed in CD11c- cells.CONCLUSION:
Cre-lox-mediated cell lineage-specific gene deletion is limited by the transient expression of recombination regulating sequences in hematopoietic cell lines. These methodological issues indicate the need to consider when to employ non-dynamic DNA recombination models in animal models of CNS autoimmunity. An experimental algorithm to address the biological complexities of non-dynamic gene recombination is provided.
Full text:
1
Database:
MEDLINE
Main subject:
Recombination, Genetic
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Integrins
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Cell Lineage
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Zebrafish Proteins
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CD11c Antigen
Limits:
Animals
Language:
En
Journal:
J Neuroimmunol
Year:
2020
Type:
Article
Affiliation country:
United States