Estrogen induces IDO expression via TGFß in chorionic villi and decidua during early stages of pregnancy.
Int J Mol Med
; 46(3): 1186-1196, 2020 Sep.
Article
in En
| MEDLINE
| ID: mdl-32583005
ABSTRACT
Indoleamine 2,3dioxygenase (IDO) is one of the most important proteins protecting the embryos from the mother's immune system during pregnancy; however, little is known about the regulation of expression of this protein at the maternalfetal interface. In the current study, chorionic villi and decidua were collected from women at early stages of pregnancy. Samples of chorionic villi and decidua were cultured in medium containing different concentrations of 17ßestradiol and estriol respectively, with or without fulvestrant. Western blot analysis and/or immunofluorescent staining were used to detect the expression of transforming growth factor ß (TGFß) and IDO in chorionic villi and decidua tissues. Both TGFß and IDO were expressed in chorionic villi and decidua. The expression levels of these two proteins increased the most in samples of chorionic villi and decidua cultured in medium containing 17ßestradiol at the concentration of 10 ng/ml, or estriol at the concentration of 1 µg/ml. This increase could be reversed when fulvestrant was added in the medium at the concentration of 10 µg/ml. IDO expression increased in a dosedependent manner in tissue samples cultured in medium containing TGFß. The results of the current study revealed that administration of estrogen at doses similar to those observed in healthy pregnant women may upregulate the expression of IDO by TGFß, suggesting that estrogen may prevent allogeneic fetal rejection and may be used as an immunomodulator.
Full text:
1
Database:
MEDLINE
Main subject:
Chorionic Villi
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Transforming Growth Factor beta
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Decidua
/
Estrogens
/
Indoleamine-Pyrrole 2,3,-Dioxygenase
Limits:
Adult
/
Female
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Humans
/
Pregnancy
Language:
En
Journal:
Int J Mol Med
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Year:
2020
Type:
Article