Quantitative evaluation of dexamethasone treatment effects in renal ischemia-reperfusion injury using contrast enhanced ultrasonography in rats.
Clin Hemorheol Microcirc
; 76(1): 99-110, 2020.
Article
in En
| MEDLINE
| ID: mdl-32651308
ABSTRACT
BACKGROUND:
Renal ischemia-reperfusion (I/R) injury often occurs in various clinical events, and its incidence and mortality have been increasing.OBJECTIVE:
To investigate the value of contrast enhanced ultrasonography (CEUS) in the monitoring of dexamethasone in the improvement of renal I/R injury in rats.METHODS:
Eighteen healthy male Sprague-Dawley rats were randomly divided into sham-operated, I/R, and I/R surgery plus dexamethasone treatment (Dexa) groups. In the I/R group 45-minute renal ischemia with 24âh reperfusion period was monitored. Time-intensity curve (TIC)-derived parameters, which included peak value, time to peak (TP), area under the curve (AUC), and mean transit time (MTT) were compared to the blood creatinine, urea, Caspase-1, and NLRP3 levels.RESULTS:
The I/R group showed an increased peak value, prolonged TP and MTT, and greater AUC (Pâ<â0.05). The Dexa group showed shorter TP and MTT, and smaller AUC (Pâ<â0.05). Results show that the associations between (i) TP, AUC, and MTT and (ii) creatinine, urea, Caspase-1, and NLRP3 levels were significant (Pâ<â0.05).CONCLUSION:
Dexamethasone can alleviate renal I/R injury in rats, which may be related to the inhibition of NLRP3 and caspase-1. CEUS can quantitatively measure this change, in which the changes in TP, AUC and MMT values have considerable reference values.Key words
Full text:
1
Database:
MEDLINE
Main subject:
Reperfusion Injury
/
Contrast Media
/
Kidney
/
Kidney Diseases
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Clin Hemorheol Microcirc
Journal subject:
ANGIOLOGIA
/
HEMATOLOGIA
Year:
2020
Type:
Article
Affiliation country:
China