Your browser doesn't support javascript.
loading
An Acid-Activatable Fluorescence Probe for Imaging Osteocytic Bone Resorption Activity in Deep Bone Cavities.
Hashimoto, Ryu; Minoshima, Masafumi; Kikuta, Junichi; Yari, Shinya; Bull, Steven D; Ishii, Masaru; Kikuchi, Kazuya.
Affiliation
  • Hashimoto R; Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Minoshima M; Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Kikuta J; Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Yari S; Immunology Frontier Research Center, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Bull SD; Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
  • Ishii M; Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
  • Kikuchi K; Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Angew Chem Int Ed Engl ; 59(47): 20996-21000, 2020 11 16.
Article in En | MEDLINE | ID: mdl-32744428
ABSTRACT
A rationally designed pH-activatable fluorescent probe (pHocas-RIS) has been used to measure localised pH levels in osteocytic lacunae in bone tissue. Conjugation of the moderate bone-binding drug risedronate to a pH-activatable BODIPY fluorophore enables the probe to penetrate osteocytic lacunae cavities that are embedded deep within the bone matrix. After injection of pHocas-RIS, any osteocytic lacunae caused by bone-resorbing osteocytes cause the probe to fluoresce in vivo, thus allowing imaging by intravital two-photon excitation microscopy. This pH responsive probe enabled the visualization of the bone mineralizing activities of acid producing osteocytes in real time, thus allowing the study of their central role in remodeling the bone-matrix in healthy and disease states.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Osteocytes / Bone Resorption / Boron Compounds / Optical Imaging / Fluorescent Dyes Limits: Animals Language: En Journal: Angew Chem Int Ed Engl Year: 2020 Type: Article Affiliation country: Japan
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Database: MEDLINE Main subject: Osteocytes / Bone Resorption / Boron Compounds / Optical Imaging / Fluorescent Dyes Limits: Animals Language: En Journal: Angew Chem Int Ed Engl Year: 2020 Type: Article Affiliation country: Japan