[Effect of Chidamide on the Killing Acitivity of NK Cells Targeting K562 Cells and Its Related Mechanism In Vitro].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 28(4): 1167-1170, 2020 Aug.
Article
in Zh
| MEDLINE
| ID: mdl-32798393
ABSTRACT
OBJECTIVE:
To investigate the effect of chidamide on the killing activity of NK (Natural killer cell, NK) cells targeting K562 cells and its related mechanism.METHODS:
K562 cells were pretreated with chidamide at different concentrations and cocultured with NK cells at different effect-target ratios. The killing effect of chidamide on K562 cells by NK cells, the expression of natural killer group 2 member D (NKG2D) ligands and apoptosis rate of K562 cells were detected by flow cytometry.RESULTS:
The killing sensitivity of NK cells to K562 cells could be enhanced by chidamide. The expression of ULBP2 on K562 cell surface could be up-regulate, however, the expression of ULBP1 and MICA/MICB showed no statistically difference as compared with control group. Chidamide showed no obvious cytotoxicity to K562 cells.CONCLUSION:
Chidamide can significantly improve killing efficiency of NK cells on K562 cells, which may be related to the up-regulation of ULBP2 expression.
Full text:
1
Database:
MEDLINE
Main subject:
Killer Cells, Natural
/
Histocompatibility Antigens Class I
Limits:
Humans
Language:
Zh
Journal:
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Journal subject:
HEMATOLOGIA
Year:
2020
Type:
Article
Affiliation country:
China