The Application of Single-Cell RNA Sequencing in Vaccinology.
J Immunol Res
; 2020: 8624963, 2020.
Article
in En
| MEDLINE
| ID: mdl-32802896
ABSTRACT
Single-cell RNA sequencing allows highly detailed profiling of cellular immune responses from limited-volume samples, advancing prospects of a new era of systems immunology. The power of single-cell RNA sequencing offers various opportunities to decipher the immune response to infectious diseases and vaccines. Here, we describe the potential uses of single-cell RNA sequencing methods in prophylactic vaccine development, concentrating on infectious diseases including COVID-19. Using examples from several diseases, we review how single-cell RNA sequencing has been used to evaluate the immunological response to different vaccine platforms and regimens. By highlighting published and unpublished single-cell RNA sequencing studies relevant to vaccinology, we discuss some general considerations how the field could be enriched with the widespread adoption of this technology.
Full text:
1
Database:
MEDLINE
Main subject:
Pneumonia, Viral
/
Viral Vaccines
/
Coronavirus Infections
/
Single-Cell Analysis
/
Pandemics
/
Betacoronavirus
/
Vaccinology
/
RNA-Seq
Limits:
Animals
/
Humans
Language:
En
Journal:
J Immunol Res
Year:
2020
Type:
Article
Affiliation country:
United kingdom