Molecular evidence of bovine herpesvirus 1 and 5 in cattle with suspected rabies in Rio Grande do Sul state, Brazil.
Comp Immunol Microbiol Infect Dis
; 73: 101495, 2020 Dec.
Article
in En
| MEDLINE
| ID: mdl-32889429
ABSTRACT
Rabies and herpetic encephalitis are the main viral infections in bovines with neurological symptoms. Bovine rabies has a high prevalence in Central and South America, while bovine encephalitis associated with herpesvirus is especially important in South America. Viral isolation is the classical way to confirm herpesvirus infection, but molecular evidence of the presence of the virus in affected animals is gaining importance in the diagnosis of the disease in the laboratory. This study investigated the presence of herpesvirus type 1 and 5 (BoHV-1 and BoHV-5) in 182 encephalon of rabies-suspected cattle in Rio Grande do Sul state (RS), Brazil using multiplex real-time polymerase chain reaction (mRT-PCR). The rabies virus was investigated by direct fluorescent antibody assay and intracerebral suckling mouse inoculation. The genomes of BoHV-1 and BoHV-5 were detected in 17% of samples. BoHV-5 and BoHV-1 were detected in 100% and 19% of BoHV positive samples, respectively, indicating the circulation of the pathogens in cattle herds in RS. The high Ct values and the absence of isolation suggest viral latency. Coinfection of herpesvirus and the rabies virus was detected in 28% of samples, although no significant association between pathogens was observed. Rabies was detected in 57.7% of suspected samples, confirming the importance of the disease in the state. Concerning the method by which samples were conserved, no significant difference was observed between the number of positive results in frozen and refrigerated samples.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Rabies
/
Cattle Diseases
/
Herpesviridae Infections
/
Herpesvirus 1, Bovine
/
Herpesvirus 5, Bovine
Type of study:
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Comp Immunol Microbiol Infect Dis
Year:
2020
Type:
Article