High-fat diet leads to elevated lipid accumulation and endoplasmic reticulum stress in oocytes, causing poor embryo development.
Reprod Fertil Dev
; 32(14): 1169-1179, 2020 Oct.
Article
in En
| MEDLINE
| ID: mdl-32998795
ABSTRACT
The present study was designed to investigate the effect of diet-induced obesity on endoplasmic reticulum (ER) stress in oocytes. Swiss albino mice (3 weeks old) were fed with a high-fat diet (HFD) for 8 weeks. Oocytes were assessed for lipid droplet accumulation, oxidative stress, ER stress and their developmental potential invitro. High lipid accumulation (P<0.01) and elevated intracellular levels of reactive oxygen species were observed in both germinal vesicle and MII oocytes of HFD-fed mice (P<0.05 and P<0.01 respectively compared with control). Further, expression of the ER stress markers X-box binding protein 1 (XBP1), glucose-regulated protein 78 (GRP78), activating transcription factor 4 (ATF4) and activating transcription factor 6 (ATF6) was significantly (P<0.001) higher in oocytes of the HFD than control group. Oocytes from HFD-fed mice exhibited poor fertilisation and blastocyst rates, a decrease in total cell number and high levels of DNA damage (P<0.01) compared with controls. In conclusion, diet-induced obesity resulted in elevated lipid levels and higher oxidative and ER stress in oocytes, which contributed to the compromised developmental potential of embryos.
Full text:
1
Database:
MEDLINE
Main subject:
Oocytes
/
Oxidative Stress
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Embryonic Development
/
Lipid Metabolism
/
Endoplasmic Reticulum Stress
Limits:
Animals
Language:
En
Journal:
Reprod Fertil Dev
Journal subject:
MEDICINA REPRODUTIVA
Year:
2020
Type:
Article
Affiliation country:
India