Forces, fluxes, and fuels: tracking mitochondrial metabolism by integrating measurements of membrane potential, respiration, and metabolites.
Am J Physiol Cell Physiol
; 320(1): C80-C91, 2021 01 01.
Article
in En
| MEDLINE
| ID: mdl-33147057
ABSTRACT
Assessing mitochondrial function in cell-based systems is a central component of metabolism research. However, the selection of an initial measurement technique may be complicated given the range of parameters that can be studied and the need to define the mitochondrial (dys)function of interest. This methods-focused review compares and contrasts the use of mitochondrial membrane potential measurements, plate-based respirometry, and metabolomics and stable isotope tracing. We demonstrate how measurements of 1) cellular substrate preference, 2) respiratory chain activity, 3) cell activation, and 4) mitochondrial biogenesis are enriched by integrating information from multiple methods. This manuscript is meant to serve as a perspective to help choose which technique might be an appropriate initial method to answer a given question, as well as provide a broad "roadmap" for designing follow-up assays to enrich datasets or resolve ambiguous results.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Organelle Biogenesis
/
Biological Assay
/
Energy Metabolism
/
Membrane Potential, Mitochondrial
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Metabolomics
/
Mitochondria
Limits:
Animals
/
Humans
Language:
En
Journal:
Am J Physiol Cell Physiol
Journal subject:
FISIOLOGIA
Year:
2021
Type:
Article