Cryo-EM Structures Reveal Transcription Initiation Steps by Yeast Mitochondrial RNA Polymerase.

De Wijngaert, Brent; Sultana, Shemaila; Singh, Anupam; Dharia, Chhaya; Vanbuel, Hans; Shen, Jiayu; Vasilchuk, Daniel; Martinez, Sergio E; Kandiah, Eaazhisai; Patel, Smita S; Das, Kalyan

De Wijngaert, Brent; Sultana, Shemaila; Singh, Anupam; Dharia, Chhaya; Vanbuel, Hans; Shen, Jiayu; Vasilchuk, Daniel; Martinez, Sergio E; Kandiah, Eaazhisai; Patel, Smita S; Das, Kalyan.

Affiliation

De Wijngaert B; Rega Institute for Medical Research, Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium.
Sultana S; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA.
Singh A; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA.
Dharia C; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA.
Vanbuel H; Rega Institute for Medical Research, Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium.
Shen J; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA.
Vasilchuk D; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA.
Martinez SE; Rega Institute for Medical Research, Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium.
Kandiah E; European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38043 Grenoble, France.
Patel SS; Department of Biochemistry and Molecular Biology, Robert Wood Johnson Medical School, Rutgers University, Piscataway, NJ 08854, USA. Electronic address: patelss@rwjms.rutgers.edu.
Das K; Rega Institute for Medical Research, Department of Microbiology, Immunology and Transplantation, KU Leuven, 3000 Leuven, Belgium. Electronic address: kalyan.das@kuleuven.be.

Mol Cell ; 81(2): 268-280.e5, 2021 01 21.

Article in En | MEDLINE | ID: mdl-33278362

Subject(s)

DNA, Mitochondrial/genetics; DNA-Directed RNA Polymerases/genetics; Mitochondria/genetics; Mitochondrial Proteins/genetics; RNA, Mitochondrial/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae/genetics; Transcription Factors/genetics; Binding Sites; Cryoelectron Microscopy; DNA, Mitochondrial/chemistry; DNA, Mitochondrial/metabolism; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/metabolism; Mitochondria/metabolism; Mitochondrial Proteins/chemistry; Mitochondrial Proteins/metabolism; Models, Molecular; Nucleotide Motifs; Promoter Regions, Genetic; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; RNA, Mitochondrial/chemistry; RNA, Mitochondrial/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/metabolism; Thermodynamics; Transcription Elongation, Genetic; Transcription Factors/chemistry; Transcription Factors/metabolism; Transcription Initiation, Genetic

Key words

MTF1; POLRMT; RNA polymerase; RNAP; RPO41; TFB2M; mitochondria; transcription factor

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Full text: 1 Database: MEDLINE Main subject: Saccharomyces cerevisiae / Transcription Factors / DNA-Directed RNA Polymerases / DNA, Mitochondrial / Saccharomyces cerevisiae Proteins / Mitochondrial Proteins / RNA, Mitochondrial / Mitochondria Type of study: Prognostic_studies Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2021 Type: Article Affiliation country: Belgium

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